PAK3KD (recombinant human PAK3 kinase domain)_价格-厂家-供应商_上海经科化学科技有限公司_蚂蚁淘

来自 : 发布时间：2025-01-31

PAK3KD (recombinant human PAK3 kinase domain)相关产品：PAK1KD, human PAK1 kinase domain PAK2KD, human PAK2 kinase domain PAK3KD, human PAK3 kinase domain PAK4KD, human PAK4 kinase domain PAK6KD, human PAK6 kinase domain PAK7KD, human PAK7 kinase domain Description:Human PAK3 kinase (UniProt: O75914; NCBI: NM_001128166) belongs to a family of p21-activated kinases. These serine/threonine kinases serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. Overexpression of PAK3 has been detected in certain types of cancer. Mutations of PAK3 have been associated with learning disability and X-linked mental retardation.

 

PAK3KD is a recombinant wild type human PAK3 kinase domain (amino acids 246-544 of full-length human PAK3) expressed in E. coli and purified to near homogeneity.Molecular Mass and Phosphorylation:The calculated mass is 33,758. PAK3KD is phosphorylated on T421 and partially phosphorylated at additional sites with mass measured at 33,835 (1P), 33,915 (2P), 33,995 (3P).Stability and Storage:Stable at -70oC for 12 months from date of receipt. Protein should be thawed on ice. Protein can be flash-frozen in liquid nitrogen and stored at -70oC.Applications:Enzymatic studies, inhibitor screen, and selectivity profilingHigh concentration available for crystallizationSequence:GPHPTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLAKPLSSLTPLIIAAKEAIKNSSR The first 4 residues GPHP are from Turbo3C cleavage site. Phosphorylated at T421.Component and Formulation:PAK3KD: 1 mg/ml in Storage Buffer of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.Activity:  Specific activity: 18,700 pmoles/min/µgAssay was done in a Kinase Buffer using 0.1 mg/ml PAKtide as a substrate with 20 μM ATP at 30oC for 30 min. The amount of ATP transferred was measured using Kinase-Glo reagent (Promega). SDS-PAGE Analysis:    温馨提示：不可用于临床治疗。

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