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当前位置: 首页 > 产品中心 > kinase > Accelagen/PAK1KD(重组人PAK1激酶结构域)/10µg/K0104S
商品详细Accelagen/PAK1KD(重组人PAK1激酶结构域)/10µg/K0104S
Accelagen/PAK1KD(重组人PAK1激酶结构域)/10µg/K0104S
Accelagen/PAK1KD(重组人PAK1激酶结构域)/10µg/K0104S
商品编号: K0104S
品牌: Accelagen Inc
市场价: ¥7500.00
美元价: 4875.00
产地: 美国(厂家直采)
公司:
产品分类: 激酶
公司分类: kinase
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
PAK1KD, Human PAK1 Kinase Domain
The Gene-to-Protein Company
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Protein PurificationBaculovirus ExpressionE. coli ExpressionMolecular BiologyProducts

Products: Kinases: PAK1KD

PAK1KD (recombinant human PAK1 kinase domain)

Catalog #:Size:Concentration: Price:
K0104S 10 µg 1 mg/ml $375
K0104M 100 µg 1 mg/ml $1,375
K0104L 1 mg 1 mg/ml $5,640
K0104B Bulk 1 mg/ml Inquiry

To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490
PRODUCT DETAILDATA SHEET
Sequence: GPHPSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMREN KNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGM DGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIA TNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH
  • The first 4 residues GPHP are from Turbo3C cleavage site.
  • Phosphorylated at T423.
Component and Formulation:

PAK1KD: 1 mg/ml in Storage Buffer of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.

Activity:
Specific activity:32,638 pmoles/min/µg

Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen):

  1. Different concentrations of PAK1KD were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 mM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
  2. Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at room temperature.
  3. Fluorescence was detected using lexc=460±40 nm and lem=528±20 nm filters.
SDS-PAGE Analysis:

Protein Purification |Baculovirus Expression | Mammalian Expression |E. coli Expression |Molecular Biology | Proteins

tel: 858-678-8618fax: 888-239-6490 toll-free: 888-365-8085email: info@accelagen.comCopyright © 2020 Accelagen Inc.All rights reserved.

品牌介绍
Accelagen是一家快速成长的基因到蛋白质公司,提供优质的服务和产品.Accelagen团队在许多项目中展示了独立的研究能力,并提供了创新的解决方案。我们的科学家在抗体、GPCRs、激酶、核受体和许多其他药物靶标和治疗蛋白的生产方面有丰富的经验。多年来,Accelagen建立了项目及时完成的无与伦比的记录。 Accelagen is a fast growing Gene-to-Protein company providing superior quality services and products. The Accelagen team has demonstrated independent research capability in many projects and provided innovative solutions. Our scientists have extensive experiences in production of antibodies, GPCRs, kinases, nuclear receptors, and many other classes of drug targets and therapeutic proteins. Accelagen has established an unsurpassed track record of timely completion of projects over the years. 品牌 货号 名称 规格 Accelagen L0110S TurboLigationTM Kit 100 rections Accelagen L0110M TurboLigationTM Kit 500 rections Accelagen L0110B TurboLigationTM Kit Bulk   Accelagen H0101S Turbo3CTM Protease (HRV 3C Protease) 2mg/ml Accelagen H0101M Turbo3CTM Protease (HRV 4C Protease) 2mg/ml Accelagen H0101B Turbo3CTM Protease (HRV 5C Protease) 2mg/ml Accelagen T0102M TurboTEVTM Protease 2mg/ml Accelagen T0102L TurboTEVTM Protease 2mg/ml Accelagen T0102B TurboTEVTM Protease 2mg/ml Accelagen N0103M TurboNucleaseTM 50,000 Unit(250 Units/ml) Accelagen N0103L TurboNucleaseTM 250,000 Unit(250 Units/ml) Accelagen N0103B TurboNucleaseTM Bulk Accelagen B0114P TurboBiotinylationTM Kit 2.5mg/ml Accelagen BO114M TurboBiotinylationTM Kit 2.5mg/ml Accelagen B0114M TurboBiotinylationTM Kit 2.5mg/ml