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Accelagen Inc(授权代理)
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当前位置: 首页 > 产品中心 > peptide > 促进剂/涡轮生物素化DTTM试剂盒/50X涡轮生物素化酶DT(5 mg)
10X涡轮生物素化混合物(5 ml)/B0112L
商品详细促进剂/涡轮生物素化DTTM试剂盒/50X涡轮生物素化酶DT(5 mg)
10X涡轮生物素化混合物(5 ml)/B0112L
促进剂/涡轮生物素化DTTM试剂盒/50X涡轮生物素化酶DT(5 mg)10X涡轮生物素化混合物(5 ml)/B0112L
促进剂/涡轮生物素化DTTM试剂盒/50X涡轮生物素化酶DT(5 mg)
10X涡轮生物素化混合物(5 ml)/B0112L
商品编号: B0112L
品牌: Accelagen Inc
市场价: ¥108000.00
美元价: 70200.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Accelagen TurboBiotinylation DT Kit, the best biotinylation kit
The Gene-to-Protein Company
Request Quote
Protein PurificationBaculovirus ExpressionMammalian ExpressionE. coli ExpressionMolecular BiologyProducts

Products: Eznyme Toolbox: TurboBiotinylation DT Kit

TurboBiotinylation DTTM Kit

Catalog #:Size:Concentration: Price:
B0112P 50X TurboBiotinylase DT (0.1 mg) 10X TurboBiotinylation Mix (1 ml) 5 mg/ml $298
B0112M 50X TurboBiotinylase DT (1 mg) 10X TurboBiotinylation Mix (1 ml) 5 mg/ml $1,678
B0112L 50X TurboBiotinylase DT (5 mg) 10X TurboBiotinylation Mix (5 ml) 5 mg/ml $5,400
T0112B Bulk 5 mg/ml Inquiry

To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490
PRODUCT DETAILDATA SHEETPROTOCOL
Components and Formulation:
  1. 50X TurboBiotinylase DT at 5 mg/ml (80 µM) in Storage Buffer of 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM TCEP, 50% glycerol.
  2. 10X TurboBiotinylation Mix (50 mM Tris-HCl, pH 7.5, 100 mM MgCl2, 100 mM ATP, 5 mM biotin)
TurboBiotinylase Purity:
TurboBiotinylase DT is a stable ultra-pure recombinant E. coli BirA biotin ligase with dual GST- and His-tags. It is purified from E. coli and has a calculated molecular weight of 62.4 kDa. It is free of protease or nuclease contamination. It can be easily removed by affinity capture through its GST- or His-tag.
TurboBiotinylase Activity:
The efficiency of biotinylation can be measured by mass spectrometry. Biotinylation adds 226.31 Da to a protein.

Alternatively, biotinylation can be evaluated by streptavidin-agarose pull-down. A Coomassie stained gel will visually show the efficiency of biotinylation.

  1. Remove biotin and ATP from the biotinylation reaction sample by a spin desalting column.
  2. Save sample as Total (T)
  3. Incubate with streptavidin-agarose for 1 hour with agitation.
  4. Spin down the streptavidin-agarose.
  5. Save the supernatant as Unbound (U)
  6. Save the streptavidin-agarose as Bound (B)
  7. Run gel and stain with Coomassie Blue.

Protein Purification |Baculovirus Expression | Mammalian Expression |E. coli Expression |Molecular Biology | Proteins

tel: 858-678-8618fax: 888-239-6490 toll-free: 888-365-8085email: info@accelagen.comCopyright © 2020 Accelagen Inc.All rights reserved.

品牌介绍
Accelagen是一家快速成长的基因到蛋白质公司,提供优质的服务和产品.Accelagen团队在许多项目中展示了独立的研究能力,并提供了创新的解决方案。我们的科学家在抗体、GPCRs、激酶、核受体和许多其他药物靶标和治疗蛋白的生产方面有丰富的经验。多年来,Accelagen建立了项目及时完成的无与伦比的记录。 Accelagen is a fast growing Gene-to-Protein company providing superior quality services and products. The Accelagen team has demonstrated independent research capability in many projects and provided innovative solutions. Our scientists have extensive experiences in production of antibodies, GPCRs, kinases, nuclear receptors, and many other classes of drug targets and therapeutic proteins. Accelagen has established an unsurpassed track record of timely completion of projects over the years. 品牌 货号 名称 规格 Accelagen L0110S TurboLigationTM Kit 100 rections Accelagen L0110M TurboLigationTM Kit 500 rections Accelagen L0110B TurboLigationTM Kit Bulk   Accelagen H0101S Turbo3CTM Protease (HRV 3C Protease) 2mg/ml Accelagen H0101M Turbo3CTM Protease (HRV 4C Protease) 2mg/ml Accelagen H0101B Turbo3CTM Protease (HRV 5C Protease) 2mg/ml Accelagen T0102M TurboTEVTM Protease 2mg/ml Accelagen T0102L TurboTEVTM Protease 2mg/ml Accelagen T0102B TurboTEVTM Protease 2mg/ml Accelagen N0103M TurboNucleaseTM 50,000 Unit(250 Units/ml) Accelagen N0103L TurboNucleaseTM 250,000 Unit(250 Units/ml) Accelagen N0103B TurboNucleaseTM Bulk Accelagen B0114P TurboBiotinylationTM Kit 2.5mg/ml Accelagen BO114M TurboBiotinylationTM Kit 2.5mg/ml Accelagen B0114M TurboBiotinylationTM Kit 2.5mg/ml